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Biobehavioral Laboratories Core

Biochemical

At present, the biochemical laboratory has the capacity to measure neurochemicals (e.g., serotonin, 5-HIAA, norepinephrine, epinephrine, dopamine), neurotransmitter synthesizing and degrading enzymes (e.g., choline acetyltransferase), and hormones (e.g., prolactin, growth hormone, testosterone, estrogen, progesterone, FSH, LH, cortisol, ACTH, melatonin). These assays are performed on various body fluids including urine, blood, and saliva from humans and on fluids and tissues from rodents. The primary biochemical methods used are high performance liquid chromatography, enzyme-linked immunoabsorbance (ELISA), chemiluminescent (Immulite), and radioimmunoassays. In addition, there is a dry chemical lab, autoclave, and 170 cubic feet of -70 degree storage space for samples for both archival and ongoing experimental studies. Lab manager Ernie Tolentino presents an overview of the Biochemical labs.

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